Heme oxygenase-1 knocked down rat mesangial cells treated with hydrogen peroxide

HO-1 cells denote the cultured rat mesangial cells with heme oxygenase-1 knocked down by RNA interference (using lentiviral vector). GFP cells denote the cultured rat mesangial cells that are transfected with empty lentiviral vector containing GFP cassette. Cells are treated with hydrogen peroxide 100 micromolar for 2 hours, or without. RNA are then harvested for array analysis. Biological replicates are performed (two independent experiment sets).HO-1细胞指通过RNA干扰（RNA interference）技术、使用慢病毒载体（lentiviral vector）敲低血红素氧合酶-1（heme oxygenase-1，HO-1）的培养大鼠系膜细胞；GFP细胞指转染了携带绿色荧光蛋白（green fluorescent protein，GFP）表达盒的空慢病毒载体的培养大鼠系膜细胞。细胞经100微摩尔（micromolar）浓度的过氧化氢（hydrogen peroxide）处理2小时，或不予处理。随后收集RNA进行基因芯片分析（array analysis），实验设置了生物学重复（biological replicates），包含两组独立实验。