A rapid assessment model for liver toxicity of macrolides and an integrative evaluation for azithromycin impurities

we report an assessment of macrolides related liver toxicity by using ADMET prediction, molecular docking, structure-toxicity relationship and experimental verification via detecting the c-fos gene expression in liver cells.We employed the model to virtual screening of azithromycin impurities with high hepatotoxicity, and then experimental confirmed by liver toxicity in zebrafish, and c-fos gene expression. Integrative evaluation for liver toxicology of azithromycin impurities in zebrafish larvae.