The plants family Ranunculaceae reproduce asexually, primarily by daughter roots, to coordinate their stress response and resource output. However, the molecular mechanism of its daughter root development remains obscure. Here, we chose Aconitum carmichaelii Debx., a typical plant of ranunculaceae that is propagated by daughter roots, to construct a transcriptome map at single-cell resolution.. Single-cell transcriptomic sequencing reveals a high-resolution gene regulatory network underlying Aconitum carmichaelii Debx. daughter roots development

The plants family Ranunculaceae reproduce asexually, primarily by daughter roots, to coordinate their stress response and resource output. However, the molecular mechanism of its daughter root development remains obscure. Here, we chose Aconitum carmichaelii Debx., a typical plant of ranunculaceae that is propagated by daughter roots, to construct a transcriptome map at single-cell resolution. We found that 单细胞结论 and proposed that ARF7 and LBD3 are important transcription factors in the daughter roots initiation. Based on PPI network and molecular docking, we showed that ARF7 mediates LBD3 co-regulation of daughter root development. By transferring ARF7 into wild-type Arabidopsis, rooting percentage and lateral root length increased 5.8 and 0.12 times, respectively, and a significant increase in the transcriptional activities of LBD3. And fluorescent protein signals indicated that ARF7 and LBD3 were expressed in the cytoplasm. In summary, our findings reveal a high-quality single-cell transcriptome profiling of A. carmichaelii, resolving and validating that ARF7 has a positive regulatory role in the development of daughter roots.