Next-generation sequencing analysis to compare expression pattern of relapse and baseline "CMP pattern" MDS samples

Transcriptional changes occurring in the expanding LT-HSC population of "CMP pattern" MDS patients with blast expansion post-HMA failure were compared with the transcriptional profile of untreated (baseline) "CMP pattern" MDS patients. CD34+CD38-CD45RA-CD90+ LT-HSCs were FACS-purified from magnetically CD34-enriched mononuclear cells isolated by density gradient from fresh bone marrow aspirates. Total RNA was purified using the PicoPure RNA Isolation Kit (Thermo Fisher Scientific). Quality checks were performed in a 2100 Expert bioanalyzer (Agilent). cDNA libraries were prepared using the Ovation Single Cell RNA-Seq System (NuGEN Technologies). RNA-seq was performed with the HiSeq 4000 System (Illumina), generating 76-base pair reads. **The submitter declares that the raw data will be deposited in dbGaP due to patient privacy concerns.**