N'-(1-phenylethylidene)-benzohydrazide cytotoxicity is LSD1 independent and linked to Fe-S cluster disruption in Ewing sarcoma

The purpose of the study was to investigate the overlap in cytotoxic and gene regulatory activity between reversible LSD1 inhibitor SP-2509 (HCI-2509) and Compounds 15, 19, and 20 which share structural similariy with SP-2509 but lack LSD1 inhibitory activity. Additionally we compared these compounds to RNAi of EWS::FLI1 (iEF) or Luciferase control (iLuc), as has been done with SP-2509, as well as with OG-L002, an irreversible LSD1 inhibitor with minimal cytotoxic activity. Overall design: RNA-seq of wildtype A673 cells treated with vehicle (DMSO), Compounds 15, 19 or 20, or irreversible LSD1 inhibitor OG-L002 (2uM) for 48 hrs. Data was combined with samples from 2 additional datasets. That data is from GSE94502 (iEF and iLuc samples) and GSE98787 (A673_DMSO and A673_HCI_2509 samples). Data exclusively from this GEO submission is located in the geo_counts.csv file, while the complete count matrix used for analysis is located in the counts.csv supplementary file. *************************************************************** The table below lists GEO accessions reused/reanalyzed for this study. ***************************************************************