Tumorigenic and metastatic properties of H460 cells and lung CSCs.
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*H460 cells and CSCs were injected s.c. into SCID mice at concentrations of 5×103–5×105 cells (in 200 µl PBS) per mouse. Mice were sacrificed when tumors reach 2 cm in diameter.**H460 cells and CSCs were inoculated i.v. into the tail vein of SCID mice (5×104 tumor cells/mouse). After 60 days mice were sacrificed, lungs were removed and fixed in the Bouin's solution, and metastatic nodules were counted under a dissecting microscope.
创建时间：
2015-12-02
