Analysis of RNA expression under AhR antagonist treatment in human HSPCs. Analysis of RNA expression under AhR antagonist treatment in human HSPCs

To examine the changes in RNA expression profile under AhR antagonist treatment in human hematopoietic stem progenitor cells (HSPCs), CD34+cells were isolated from umbilical cord blood (UCB) unit. CD34+cells were cultured with two different AhR antagonists CH223191 and StemRegenin1 (SR1) ex vivo for 7 days. cultured cells were used to perform RNA sequencing and analyze the changes in RNA expression profile by treatment. Overall design: Examination of 2 different Ary hydrocarbon receptor antagonists in cord blood (CB)-HSPCs (DMSO control vs. CH223191-treated vs. StemRegenin1-treated). Three biological replication of each group from identical individuals