Y-27632 Enhances Cell Migration in Wound Healing Assay.

(A) Representative phase-contrast microscopy images of CSC migration and time-course of wound healing after 10μM Y-27632 treatment for 48hrs. The “Wound Sizes” were measured by determining the means of at least 3 points along each edge as indicated with white arrows (a3 to a10). Scale bar = 500μm. The relative “wound sizes (%)” of Control (filled circle) and Y-27632-treated (filled square) cells were compared at 0, 2, 6, 12, & 24hrs post-wounding by normalizing to 0hr. ns: no significance, *: pp(B) Phalloidin staining to evaluate formation of cellular F-actin filaments after treatment with 10μM Y-27632 for 48hrs. The representative fluorescent microscopy images display F-actin in green (Phalloidin) and nuclei in blue (Hoechst). Scale bar = 200μm. The means of relative fluorescence intensities per image were analyzed and plotted as an insert in Fig B, c5 (*: p