Myosin-dependent short actin filaments contribute to peripheral widening in developing stereocilia

Airyscan microscopy images: Fig. 1b_Live imaging of EGFP-actin Fig. 2a-b_RPEL1-EGFP Fig. 2c-e_AC15 or JLA20 labeling Fig. 3a-c_EGFP-TMOD1 Fig. 6_mutant actin_AP or DVD Fig. 7b_PE in myo3ab mutant Fig. 7d-e_PE in myo15a mutant Suppl. 1_early postnatal day OE and prolonged OE Suppl. 4b-d_PE or BE label in EGFP-actin IHC Suppl. 5a-b_vanadate treatment Suppl. 6e_MYO3A staining in myo15a null Expansion microscopy images: Fig. 1c_EGFP-actin incorporation pattern Fig. 7a_Localization of His-TMOD1_MYO3A_MYO15A Suppl. 7b_Stereocilia width in EGFP-MYO3A OE IHCs Lattice SIM images: Fig. 1d_EGFP-actin for FSCN2 pattern Fig. 3f-h_BE or PE localization Suppl. 5c-d_MYO3A or MYO15A localization Leica SP8 images: Fig. 3d_PE or BE probing Fig. 4a-b_His-TMOD1 level_LatA Fig. 4c-f_Postnatal His-TMOD1 labeling_P3-P9 Fig. 5_Stereocilia widen_PE_BE Fig. 7c_PE in MYO3 mutants Fig. 7f-g_PE level_OE of MYO3A Fig. 7h-i_PE level_OE of MYO15A Suppl. 2_EGFP-SH3BGRL2 Suppl. 3_PE or BE probing_high salt Suppl. 4a_Actin-IRES-EGFP Suppl. 6c_MYO15A and PE in myo15a mosaic IHCs Suppl. 6f_MYO15A stain in myo3 mutant Suppl. 7a_OE of EGFP-MYO3A-WT Nikon Ti2-E CSU-X1 images: Suppl. 6d_His-TMOD1 probing in shaker IHCs