Bulk RNA-seq study 4 and 5: 17 genes knocked out in extra-embryonic and cortical brain organoids

This study investigates the effects of knocking out 17 genes - DMRTA1, TBR1, OVOL1, PITX1, LMO2, ZBTB7C, HEY1, MBNL2, DLX4, DLX5, DLX6, HOPX, MAFF, TWIST2, MBNL3, SIX3 and NR2F1, by inserting premature termination codons (PTCs) in primitive syncytium and cortical brain organoid model systems derived from the KOLF2.2J iPSC line. Bulk RNA-seq analysis was performed to examine the transcriptional consequences of these gene knockouts, providing insights into their roles in brain development and cellular differentiation in organoid models Refer to individual Series