Genomic analysis of a riboflavin-overproducing Eremothecium gossypii (Ashbya gossypii) mutant isolated by disparity mutagenesis

Eremothecium gossypii (Ashbya gossypii) naturally overproduces riboflavin and has been utilized for industrial riboflavin production. Regarding, a riboflavin-overproducing mutant isolated by disparity mutagenesis, its genomic analysis was carried out. In the genomic analysis, 33 homozygous and 1377 heterozygous mutations were detected. Among these heterozygous mutations, the proportion of mutated reads in each gene was different, ranging from 21 to 75%. This study provides new insights into riboflavin production in this fungus and the usefulness of disparity mutagenesis for the creation of new types of mutants for metabolic engineering.