Advancing airborne eDNA sampling results in robust characterisation of vertebrate communities

These data files contains the multiplexed sequence files and bioinformatic pipelines used in “Advanced airborne eDNA sampling allows robust spatiotemporal characterisation of vertebrate communities”. Brief methods This study include sequences related to 3 different experiments. In each experiment, for each sample (and the blank and positive samples) we have conducted six PCR replicates, where PCR products were barcoded during the first PCR. We used 7bp barcodes (tags) in 5’ of forward and reverse primers to barcode the samples (Bohmann et al. 2022) and barcode combinations are not repeated within each set of PCR replicates. This allows us to pool the samples after the first PCR and build libraries on the purified amplicon pool. Each amplicon pool was used to build dual-indexed Illumina libraries using the PCR-free TagSteady protocol (Carøe and Bohmann 2020). Libraries were sequenced on an Illumina MiSeq instrument (paired-end 150bp (V3)) at the GeoGenetics Sequencing Core, University of Copenhagen, Denmark. Sequence files given here are for each amplicon pool and “Tag.txt” file can be used to demultiplex the samples.