Next Generation Sequencing Facilitates Quantitative Analysis of ISL1 Regulated Transcriptomes

The goal of this study is to analyze the transcriptome profiling (RNA-seq) after ISL1 knockdown. The objective of this study is to elucidate the mechanisms of ISL1 in gastric carcinogenesis, RNA-Seq is performed in BGC823 cells with stable ISL1 knockdown. Comparison and identification of the candidate genes based on the intersection of RNA-seq showed that upregulated and downregulated genes had ISL1-bound regions. Overall design: BGC823 cells were transfected by ISL1 knockdown lentivirus and scramble lentivirus as control.Total RNA was extraced by Qiagen kit and the mRNA profiles were generated by deep sequencing, in duplicate, using Illumina Hiseq 2000.