Additional file 12: of Assessing characteristics of RNA amplification methods for single cell RNA sequencing

Normalized read counts and expectation for ERCC transcripts. A–B. ERCC transcripts are found along the x-axis, ordered by expected number of input molecules. Axis labels are in the format of “ERCC spike-in ID, expected number of input molecules”. Points indicate the normalized read count for one transcript in one sample. Horizontal gray lines and background gray boxes indicate the expected normalized read count and a 95% CI under a Poisson model of dilution. Wide red horizontal lines indicate mean normalized read counts across all ERCC transcripts with a common expected number of input molecule, and red boxes indicate mean ± 2 × s.e.m. (A) aRNA. (B) SmartSeq Plus. (XLS 964 kb)