Staygreen trait mapping. Exome capture data

Differentially senescing Triticum aesticum cv Paragon EMS mutants were identified during a forward genetic screen. RIL populations segregating for senescence traits were developed for trait mapping purposes by crossing each mutant to parental cv. Paragon and advanced to the F4 generation. Following multiple years of in-field phenotyping RILs were classified as displaying 'staygreen' or 'non-staygreen' phenotypes and selected for inclusion in bulks. Gene-encoding regions of staygreen mutant parents, parental cv. Paragon and contrasting bulks were sequenced using exome-capture technology (SeqCapEZ probe set 140430_Wheat_TGAC_D14_REZ_HX1 for Triticum aestivum (Roche, Nimblegen, WI, USA; Krasileva et al., 2017), with resulting analysis enabling trait mapping.