Novel Propolis-Aloe Vera Nanofilms with Antimicrobial, Anti-inflammatory and Pro-Angiogenic Activity for Acute Wound Healing

To observe the effect of nanopore size rapid film (NRF) co-loaded with propolis and aloe vera extract in the treatment of wound healing. Methods: The porosity, mechanical properties, and water vapor transmittance rate (WVTR) of the materials were determined, and the effect of P-A-NRF in promoting cell proliferation and migration was evaluated, and the mechanism of wound healing in a mouse full-thickness wound model was provided. Results: P-A-NRF is rapidly film-forming within 5min, and the contact angle is 80°, which has a waterproof effect. The cell viability rate of P-A-NRF was more than 80%, which was significantly higher than that of the positive control group, and the cell migration rate reached more than 50%. In vivo experiments, the P-A-NRF group achieved a 98% healing rate at 14 days 90% in the commercially available liquid band-aid group, and 70% in the blank control group, with faster wound healing. The expression levels of IL-6, IL-1β, TNF-α, VEGF, and Hydroxyproline were measured by enzyme-linked immunoassay (ELISA), the expression levels of IL-6, IL-1β and TNF-α in the P-A-NRF group were significantly decreased compared with those in the control group and the commercially available liquid band-aid group, and the levels of VEGF and Hydroxyproline were increased. Conclusion: The film formed by P-A-NRF is uniform, soft, and malleable. It can enhance cell viability, promote cell proliferation and migration, and accelerate wound healing mainly by reducing inflammation, antibacterial, and promoting angiogenesis and collagen deposition, which is a promising wound dressing.