Effects of Rny1 mutants on growth and tRNA cleavage.

Results of analysis of deletions to regions of Rny1 and alanine mutagenesis of loops 2–11 (L2–L11). Exact mutations are indicated for each construct. Growth was assayed by over-expression in the hir2Δ strain, using the rny1-ci background, on plates containing galactose. All strains were tested and grew equivalently on dextrose. Cleavage of tRNA was tested by harvesting rny1Δcells over-expressing the indicated constructs, resolving equivalent amounts of their total RNA by gel electrophoresis, and probing by Northern blot for tRNA Met(CAT). Additional details can be found within Materials and Methods. NT = not tested. aa = amino acids.