Murine intestinal organoids resemble intestinal epithelium in their microRNA profiles

To compare miRNA expression profiles between freshly isolated intestinal epithelial cells and cultured organoids in mice. Intestinal organoids largely resembled intestinal epithelial cells in their miRNA profiles. Although the expression levels of some miRNAs were different between crypt and villus epithelial cells, such expression patterns were not reproduced during the maturation of intestinal organoids. miRNA expression levels in the crypt and villus epithelial cells isolated from murine small intestine and in the organoids cultured from murine small intestinal crypts were analyzed by microarray.