Cisplatin resistance screening in KB2P 2D cell lines

BRCA1 and BRCA2 both function in DNA double-strand break repair by homologous recombination (HR). Due to their HR defect, BRCA1/2-deficient cancers are sensitive to DNA damaging agents but they eventually acquire resistance. Preclinical studies yielded several resistance mechanisms that do not involve BRCA1/2 reactivation, but their relevance in the clinic remains elusive. To investigate which BRCA1/2-independent mechanisms drive spontaneous resistance in vivo, we performed loss-of-function screens targetting the Whole Genome.