TIRF, dSTORM and PREM images supporting data in figure 2 of "The nanoscale molecular morphology of docked exocytic dense-core vesicles in neuroendocrine cells"

This dataset is part of a collection of imaging data (https://doi.org/10.25444/nhlbi.c.5405490) from the publication entitled: "The nanoscale molecular morphology of docked exocytic dense-core vesicles in neuroendocrine cells." by Prasai et al. 2021 (https://doi.org/10.1038/s41467-021-24167-9). <br>Figure 2 contains the source of the representative correlative superresolution light and electron microscopy (CLEM) images shown in fig.2a-e. And, it contains all the underlying images that were used in generating fluorescence profiles for dGFP-Rab3a, Rab27a, Rabphilin3a, Granuphilin, and Anti-Rim2 in figure 2h.<br>For CLEM, PC12 cells were were maintained in growth media containing DMEM (Life Technologies), 10% fetal bovine serum (Life Technologies 26140-079), and 1% vol/vol penicillin/streptomycin. They were transiently transfected with DCV marker NPY-mNG and dGFP tagged proteins of interests. Cells were unroofed, fixed, labeled with Alexafluor 647 nanobody, and imaged with Nikon NSTORM microscope for dSTORM. Fluorescently imaged samples were prepared for EM that included fixation in 2% glutaraldehyde, staining with tannic acid and uranyl acetate, serial dehydration with ethanol, critical point drying, metal coating, replica lifting, and imaging with JEOL 1400 equipped with SerialEM for montaging. Processed dSTORM and EM images were correlated using an affine spatial transformation with nearest-neighbor interpolation to map the Gaussian centers of gold nanorods visible in both dSTORM and EM images.