Next Generation Sequencing Facilitates Quantitative Analysis of Honey Bee Brain Transcriptomes Treated by Dinotefuran

Purpose:The goals of this study are to obtain NGS-derived honey bee brain transcriptome profiling (RNA-seq) after dinotefuran treatment in honey bee Methods:Through deep sequencing in triplicate, using Illumina Hiseq2500,a comprehensive analysis of lncRNAs and mRNAs was performed following persistent exposure to 0.01 mg/L dinotefuran for 1, 5, and 10 d. Results: In total, 312 lncRNAs and 1341 mRNAs, 347 lncRNAs and 1458 mRNAs, 345 lncRNAs and 1155 mRNAs were found to be differentially expressed (DE) on days 1, 5 and 10 respectively. Conlusions:This study characterized the expression profile of lncRNAs upon exposure to neonicotinoid insecticides in young adult honey bees. Overall design: Honey bee brain lncRNA profiles of 1-day old, 5-day-old and 10-day-old dinotefuran treated groups and control groups