Neutralizing antibody titers in mice immunized with D1ED III formulated with various adjuvants1.

1Mice were immunized with D1ED III (10 µg/dose) formulated with the indicated adjuvants three times at two-week intervals. Serum samples (n = 5) were collected on 2 weeks after the last immunization. The dengue-1 virus neutralizing capacity was determined by FRNT. The neutralizing antibody titer was calculated as the reciprocal of the highest dilution that resulted in a 50% or 80% reduction of FFU compared to control samples containing the virus alone.2Statistical analysis was performed by the ANOVA Bonferroni post test.*p#p§p†p