Evaluation of immunological adjuvant activities of saponin rich fraction from the fruits of <i>Asparagus adscendens</i> Roxb. with less adverse reactions

The hemolytic activity, <i>in vitro</i> as well as <i>in vivo</i> toxicity, and immunomodulatory potential of saponins-rich fraction of <i>Asparagus adscendens</i> Roxb. fruit (AA-SRF) have been assessed in this study in order to explore AA-SRF as an alternative safer adjuvant to standard Quil-A saponin. The AA-SRF showed lower hemolytic activity (HD₅₀ = 301.01 ± 1.63 µg/ml) than Quil-A (HD₅₀ = 17.15 ± 2.12 µg/ml). The sulforhodamine B assay also revealed that AA-SRF was less toxic to VERO cells (IC₅₀≥200 ± 4.32 µg/ml) than Quil-A (IC₅₀ = 60 ± 2.78 µg/ml). The AA-SRF did not lead to mortality in mice up to 1.6 mg and was much safer than Quil-A for <i>in vivo</i> use. Conversely, mice were subcutaneously immunized with OVA 100 μg alone or along with Alum (200 μg) or Quil-A (10 μg) or AA-SRF (50 μg/100 μg/200 μg) on days 0 and 14. The AA-SRF at 100 μg dose best supported the LPS/Con A primed splenocyte proliferation activity, elevated the serum OVA-specific total IgG antibody, IL-12, CD4 titer and upsurged CD3/CD19 expression in spleen as well as lymph node sections which in turn advocated its adjuvant potential. Thus, AA-SRF can be further studied for use as a safe alternative adjuvant in vaccines.