Single-cell sequencing reveals heterogeneity effects of arsenic or/and 2,2-dichloroacetamide on zebrafish liver

Arsenic and DBPs has been found to be one of the major risk in many regions of the world. However, current understanding of thier combined toxicities are unclear. Here we used single-cell RNA sequencing to provide the transcriptome heterogeneity of 13563 liver cells obtained from zebrafishes exposed to 100µg/L arsenic, 300µg/L dichloroacetanilide (DCAcAm) and co-exposure for 23 days. Five liver cell populations were identified. We found that the hepatocytes and macrophages were the main target of arsenic and DCAcAm exposure. And the hepatocytes of male and female showed a huge difference, when expsoure to arsenic and DCAcAm). Overall design: Single-cell RNA sequencing were adopted to learn the transcriptional variation in zebrafish liver exposure to arsenic and DCAcAm.