New Age of Fishes initiated by the Cretaceous−Paleogene mass extinction

Samples were processed using a standard ImageJ protocol to clean the images and find individual light "particles" on a dark background. Due to their often semi-transparent nature, often teeth had to be "filled in" to ensure that ImageJ found their correct borders. This was done iteratively and by hand, until all objects visible in the image were captured by the "Analyze Particles" function corretly.