Interplay of vascular endothelial growth factor receptors in organ-specific endothelial cell survival

The aim was to study immediate changes in gene expression of lung and heart endothelial cells after single or compound deletions of vascular endothelial growth factors. Overall design: scRNA sequencing analysis of murine pulmonary and cardiac endothelial cells deleted of vascular endothelial growth factor receptors for 48 hours. Deletions include i) single deletions of Vegfr1, Vegfr2, and Vegfr3; ii) double deletions Vegfr1;Vegfr2 and Vegfr2;Vegfr3, and iii) triple deletion of Vegfr1;Vegfr2;Vegfr3. Naming of the files: a) C69, C72 etc indicates running number of experimental cohorts. R1=VEGFR1, R2=VEGFR2 and R3=VEGFR3. Ctrl samples are collected from tamoxifen treated littermate controls, Del samples had Cdh5-BAC-CreERT2. In each Ctrl and Del sample, equal amounts of sorted cells from 2-3 mice are pooled.