Prediction of broadly neutralizing antibodies [BD Rhapsody]

We generated the humoral immune repertoire of circulating memory B cells from healthy and HIV infected immune donors. These repertoires were used to predict specific HIV antibodies directed against gp120/gp41. Overall design: Peripheral blood mononuclear cells (PBMC) were isolated from a pool of HIV infected donors. Single-cell BCR repertoire sequencing of CD19+ cells was performed using BD Rhapsody Single-Cell Analysis System from BD Biosciences.