Comparison between naïve, tolerant and memory CD8+ T cells. Mus musculus

To elucidate transcriptional differences between naïve, tolerant and memory CD8+ T cells, genome-wide gene expression profiling was performed. The indicated cell populations were purified from immunized or naïve Rag-/- TCR mice by cell sorting and RNA was extracted labeled and hybridized to an Affymetrix custom mouse array GNF1M GeneChip. RNA from sorted samples was prepared, split into two aliquots prior to making labeled cDNA, so that two arrays were used for each cell population.Alterations in transcript levels were determined through pair wise comparisons of naïve cells to either memory or tolerant CD8+ T cells. Keywords: cell type Overall design: duplicates consisted of replicate aliquots of the same total RNA