Supplementary Data for "No evidence of light inhibition on aerobic methanotrophs in coastal sediments using eDNA and eRNA"

Supplementary Data files for the study "No evidence of light inhibition on aerobic methanotrophs in coastal sediments using eDNA and eRNA".

Captions to supplementary data files 

Supplementary Data 1 The data file shows the taxonomy and read counts after processing the samples with the DADA 2 pipeline. Sample labels denote: S = Shallow site, D = Deep site, M = Medium light treatment, H = High light treatment. The numbers denote the sediment core number. A few samples had < 5000 read counts and were excluded from the analysis (marked with an x in the sample name).

Supplementary Data 2 Details of the total RNA-seq including sequencing facility identifiers, sample names, and number of sequences obtained before and after quality trimming etc.

Supplementary Data 3 CH4 concentration, organic matter (%), and light intensity measured for each sample. CH4 concentrations and organic matter were measured in the top 0-1 cm sediment layer. Light intensity was measured at each location in the incubation chamber where each sample was placed.

Supplementary Data 4 Sheet 1 shows the raw results from DADA 2 including all ASVs with read counts. Sheet 2 shows the relative abundance (%) of groups within known methanotrophic lineages.

Supplementary Data 5 Sheet 1 shows reads counts from Kraken2+Bracken2 classifications of the extracted 16S rRNA sequences from the total RNA-seq data. Sheet 2 shows reads counts for groups within known methanotrophic lineages.

Supplementary Data 6 Sheet 1 and sheet 2 shows the DIAMOND+MEGAN KEGG classifications of the non-rRNA reads, sheet 1 shows the number of annotated counts per KEGG KO, while sheet 2 shows the same data but normalized as counts per million reads (CPM). Sheet 3 shows the results of the separation of amoAB-pmoAB hits by extracting these reads from MEGAN, followed by UniProtKB classification using BLASTX. The sheet shows the results in counts. Sheet 4 shows the sum of CPM values for KEGG KOs within the KEGG category Photosynthesis.