Gene Expression Analysis of RBM5 KO in Brain. Gene Expression Analysis of RBM5 KO in Brain

This study analyzed the effect of RBM5 gene deletion in cortical brain tissue on differential gene expression/splicing changes 48h after a traumatic brain injury (TBI). TBI was induced in WT vs. KO mice by controlled cortical impact (CCI) injury. The four grouops included: (1) Sham-WT, (2) CCI-WT, (3) Sham-KO, and (4) CCI-KO. The objective of this study was to test if RBM5 KO decreased the expression of cell death mediators in the contused brain 48h post-injury. Overall design: A total of 28 brain tissues were isolated from 12-15wk old mice for microarray analysis. Seven animals were compared per group (n=7/group). Each group contained a mix of female and male mice as indicated for each sample in the list below. Injured mice recieved a craniotomy over the left parietal cortex via a dental drill, and subjected to a CCI injury (6m/s; 1.2mm depth). Control (sham) mice received anesthesia but did not get a craniotomy. 48h after the injury mice were euthanized and cortical tissues collected for total RNA extraction. Extracted RNA was hybridized to microarray chips (MTA 1.0).