16S rRNA next generation sequencing of bacterial community from Tama River

In an effort to determine genus- or species-level taxonomic profiles and diversity of bacterial consortia in the Tama River around urban Tokyo, next generation sequencing (NGS) technology targeting 16S rRNA gene amplicon was employed. Metagenomic analysis was performed by Ion PGM and MiSeq after sequentially filtering samples through 5, 0.8 and 0.2 ??m.