Effects of hyperbaric oxygen on tissue-specific aging and senescence in mice and human stromal cells

This study investigates the effects of hyperbaric oxygen (HBO) on cellular senescence and aging-related phenotypes. In naturally aged mice, HBO treatment reduced systemic senescence burden and reversed aging-related transcriptomic signatures in skin, liver, and kidney tissues. Additionally, single-session HBO exposure was shown to preferentially suppress SASP propagation in human stromal cells. This dataset includes transcriptomic profiles of skin, liver, and kidney tissues from young, aged, and HBO-treated aged mice, as well as human primary prostate stromal cells (PSC27). Overall design: Total RNA was extracted from frozen tissues or cultured cells. Transcriptomic profiles were generated using the Illumina platform. For mouse tissues, RNA was extracted from skin, kidney, and liver tissues of young (2 months), aged (21 months), and HBO-treated aged mice (21 months). For HBO treatment, aged male mice were exposed to 2.0 ATA HBO for one month. Sham-treated aged mice and 2-month-old young mice served as controls. For cultured cells, RNA was extracted from bleomycin-induced senescent human primary prostate stromal cells with or without single-session 2.0 ATA HBO, with proliferative cells serving as controls.