Role of CITED2 in shaping macrophage inflammatory response

CBP/p300-interacting transactivator with glutamic acid/aspartic acid-rich carboxyl-terminal domain 2 (CITED2) is a member of the CITED family of proteins that play very critical roles in cellular development and differentiation. Several independent studies have revealed that mutation in the CITED2 gene is associated with human congenital heart defects. Concordant with these observations, experimental murine studies affirmed that mutation or loss of Cited2 resulted in congenital heart defects, as well as perturbation in the left-right patterning of the body axis. Furthermore, deficiency of Cited2 significantly impaired the development of lung, liver, placenta, and adrenal tissue at the embryonic stage (PMCID: PMC5809687). Overall design: Here we isolated bone marrow of 3 mice per group (Lyz2cre and Lyz2cre:CITED2Fl/Fl) and differentiated into macrophages by culturing them in M-CSF containing media. These cells were stimulated with 100ng/ml LPS for 4hours, 10ng/ml IFNg for 6 hours or 20ng/ml IL4 for 18 hours. Total RNA was isolated and subjected to RNAseq analyses.