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Transcriptome analysis of CD45-CD31+ endothelial cells in ischemic hindlimb muscle after IL-35 injection

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NIAID Data Ecosystem2026-03-12 收录
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https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE155012
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资源简介:
We report the first time of the transcriptome difference between IL35- and PBS-injected ischemic muscle after hindlimb ischemia, specifically in isolated CD45-CD31+ endothelial cell After single cell suspension, we specifically isolated CD45-CD31+ bona fide EC from the ischemic muscle at day 0, and 14 after hindlimb ischemia surgery using fluorescence-activated cell sorting (FACS). About 0.1 million cells were collected for RNA extraction and deep-sequencing. Single-end reads at 75 bp with 30 million reads per sample were generated for bioinformatic analysis.
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2020-11-09
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