The metabolic effects of osteopontin-c depend on the splice junction.

The metabolic effects of osteopontin-c depend on the splice junction. We stably transfected MCF-7 cells with OPNc versus OPNcM3. Expression was confirmed by Western blotting [44]. MCF-7 cells transfected with osteopontin-c or its mutant M3 were plated in poly-HEMA coated wells. After 48 hours, the cells were harvested and lysed for analysis of the metabolite levels by NMR. Shown are the fold-change and p-value for the steady state-levels of metabolites in osteopontin-c transfectants (C) versus mutant transfectants (M3).The metabolic effects of osteopontin-c depend on the splice junction.