C3bot1E174Q-C2I ADP-ribosylates actin in vitro.
收藏Figshare2016-02-24 更新2026-04-29 收录
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https://figshare.com/articles/dataset/_C3bot1E174Q_C2I_ADP_ribosylates_actin_in_vitro_/174614
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Lysate from J774A.1 cells (20 µg) was either left untreated for control (con) or incubated for 10 min at 37°C with either C3bot1E174Q-C2I (0.1 ng/mL, 0.5 ng/mL, 1 ng/mL, 10 ng/mL) or C2I (0.1 ng/mL, 0.5 ng/mL, 1 ng/mL, 10 ng/mL) in the presence of biotin-NAD+ (10 µM). Samples were subjected to SDS-PAGE, blotted and biotinylated (i.e. ADP-ribosylated) actin was detected with streptavidin-peroxidase.
创建时间：
2016-02-24
