<b>Chromosome-level genome assembly of the tetraploid Chinese cherry (</b><b><i>Prunus</i></b><b> </b><b><i>pseudocerasus</i></b><b>)</b>

we present a high-quality chromosome-level genome assembly for Chinese cherry 'Duiying'. The high-quality genome of 'Duiying' was obtained via Illumina, Pacific Biosciences (PacBio) high-fidelity (HiFi), and BioNano sequencing combined with 10× genomic and high-throughput/resolution chromosome conformation capture (Hi-C) technologies. The genome sequence of P. pseudocerasus 'Duiying' reported here will be a valuable resource for genetic studies and breeding programs in cherry plants, both for exploring the genome evolution of Rosaceae/ Prunus, and the full use of its excellent trait gene resources.