Supplemental Material for Yang et al., 2021

Supplementary File S1. This file. Supplemental methods and results.<br>Supplementary File S2. Sequence and annotation for the plasmid used to generate the phiC31 landing pad via MosSCI. SnapGene compatible file. <br>Supplementary File S3. PhiC31 recombination mediated cassette exchange (RMCE) protocol. <br>Supplementary File S4. Fasta file containing 7 sequences: attB_retrofitted_Sinv47D10_short (original and partially corrected) and attB_retrofitted_Sinv47D10 (original and partially corrected) BACs as well as the LG II sequences, which carry the antIs31, antIs32, and antIs33 sequences, for BRC0566, BRC0906, and BRC0882, respectively.<br><br><br>Supplementary Figure S1. Construction of pCG150_double_phiC31_attB plasmid.<br>Supplementary Figure S2. Design of dual color operon reporters.<br>Supplementary Figure S3. Ladder PCR of single-copy insertions of transgenes.<br>Supplementary Figure S4. Confirmation that phiC31 expressed from the antIs30 transgene is spliced.<br>Supplementary Figure S5. phiC31 integrase mediated RMCE using antIs30 in C. elegans.<br>Supplementary Figure S6. Validation of 33.4 kb BAC integration.<br>Supplementary Figure S7. Large BAC integration attempt.<br><br><br>Supplementary Table S1. Strains used and generated in this study.<br>Supplementary Table S2. Information for RMCE and other plasmids used in this study.<br>Supplementary Table S3. Primers used in this study and associated important sequences.<br>Supplementary Table S4. PCR assay results for candidate insertion lines derived from the longer BAC, attB_retrofitted_Si47D10<br>