Quantitative profiling of BATF family proteins/JUNB/IRF hetero-trimers using Spec-seq. synthetic construct
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https://www.ncbi.nlm.nih.gov/bioproject/PRJNA397060
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资源简介：
Spec-seq directly mesaures specificity by sequencing. We run an EMSA experiment on TF of interest with a library of DNA targets. Then we extract and sequence the bound and unbound portion of the DNA separately. Finally we count the occurences of each varient in the library and calculate the ratio and the relative binding energy. Overall design: 15 independent Spec-seq experiments
创建时间：
2017-08-03
