transcriptome sequencing of two different scions following grafting

Two different cultivars 'Pawnee' and 'Jinhua' were used as scion, and pecan seedlings as rootstock. The two different cultivars were grafted seperately using patch budding method. Scions were collected for sequencing following grafting. We aimed to study the transcriptome differences between eazy-to-surivive ('Pawnee') and hard-to-survive ('Jinhua') cultivars when sujuected to budding.