Exercise promotes skeletal muscle growth in adolescents via modulating Mettl3-mediated m6A methylation of MyoD in muscle satellite cells

Exercise promoted skeletal muscle growth in adolescents through facilitating MyoD mRNA stability of MuSCs in a Mettl3-mediated m6A dependent manner. The methyl donor betaine could be a potential alternative to exercise for promoting adolescent skeletal muscle growth by directly augmenting the global levels of m6A methylation. These findings may provide a theoretical foundation for encouraging daily fitness exercise and ensuring healthy growth in adolescents. To investigate the underlying mechanisms governing the effects of exercise on adolescent skeletal muscle growth, we conducted RNA-seq comparing TA from exercised mice with those from control mice. The exercise model was established in accordance with a modified protocol as described [24, 25]. Briefly, mice underwent a familiarization phase on the treadmill for 5 days, and then the speed was gradually increased from 8m/min to 12m/min, for 30 minutes daily. Subsequently, the mice were subjected to four weeks of treadmill training at a speed of 12m/min for 45min/day, 5 days/week.