Transcriptome sequencing of K562 cells stably expressing SUMO negative MYB

We expanded our previously reported transcriptome profiling of K-562 cells (GSE85187) by analyzing the global transcriptional change upon rescue of endogenous MYB knock down (KD) with SUMO-conjugation deficient mutant of MYB (2KR-MYB) and SUMO-binding deficient mutant of MYB (ANAA-MYB) in comparison with K-562 cells treated with siGENOME non-targeting siRNA (GSE85187) , endogenous MYB KD treated with si2992 (GSE85187), rescue of endogenous MYB KD with wild-type MYB (GSE85187) and rescue of endogenous MYB KD with D152V mutant of MYB (GSE85187). Cells were tranfected with siRNA and 24 hours after transfection total RNA was extracted. Three biological replicates were used for RNA sequencing, where 150 bp PE-reads were obtained using Illumina Hiseq 4000 sequencer