RNA velocity analysis of the PERFORM RNA-Seq dataset

For our RNA velocity study we used whole-blood RNA-Seq data generated from a 399 subset (patients with suspected infection) of the Personalized Risk assessment in Febrile illness to Optimize Real-life Management across the European Union (PERFORM) study cohort - a multi-country prospective observational study of children with suspected infection presenting to hospitals in Europe. We also included 19 non-infection control subjects recruited to PERFORM, predominantly from out-patient settings, who were well at the time of attendance and the reason for attendance was unlikely to affect the blood transcriptome, for example pre-surgical assessment for polydactyly or tonsillectomy. Total RNA was isolated using PAXgene miRNA blood extraction kits (Qiagen), and after additional DNAse treatment (Zymo Research), was sent for ribodepletion library preparation and RNA-Seq at The Wellcome Centre for Human Genetics in Oxford, United Kingdom, using a Novaseq6000 platform at 150bp paired-end configuration, generating a raw read count of ~30 million reads per sample. Samples are from paired end data (_1 and _2). Clinical events of the same person have the same number (PERFORM_number). The event number is specified under the E part of the sample name. Timepoint is specified by TP. All samples in this submission are timepoint 1.