Expression data of human NK cell treated with lenalidomide

Lenalidomide, an analogue of thalidomide, is immuno-modulatory drug and has been used for many types of cancer therapy. Recently, it has been reported that lenalidomide can enhance the function of NK cell during IL-2 response. However, molecular mechanism of action of lenalidomide in NK cells is unclear still. To investigate the modification by lenalidomide on NK cells, we conducted the analysis of gene expression on NK cells. PBMCs were isolated from 2 healthy donors using by Ficoll-Hypaque density gradient centrifugation on Histopaque-1077 (Sigma-Aldrich). PBMCs were cultured with DMSO or 1uM lenalidomide plus 200U/ml IL-2 for 12 days. Human NK cell separated by NK cell isolation kit (Miltenyi Biotec) on day 12. NK cell purity was at least 85% and used for microarray analysis