Identify genes involved in ER stress in wheat using RNA sequencing

We used dithiothreitol (DTT) and tauroursodeoxycholic acid (TUDCA) to induce or suppress ER stress in wheat cells, respectively, with the aim to reveal the molecular background of ER stress responses using RNA sequencing. Transcriptomic analysis revealed that 8204 genes were differentially expressed in three treatment groups. Among these genes, 158 photosynthesis-related genes, 42 antioxidant enzyme genes, 318 plant hormone-related genes and 457 transcription factors (TFs) might play vital roles in regulating wheat response to ER stress. Overall design: Experiments were established with three treatments: control (C), DTT (D), and DTT+TUDCA (T). Three independent biological replicates were performed, and a total of nine leaf samples were performed by RNA sequencing.