Supplementary information_Anti-mycobacterial Potential of Endophytic Fungi from West Sulawest, Indonesia, against Mycobacterium tuberculosis.

This study investigated the anti-mycobacterial potential of endophytic fungi isolated from medicinal and endemic plants collected in West Sulawesi, Indonesia. Endophytic fungi were obtained from surface-sterilized plant tissues and cultured under laboratory conditions. Crude extracts were prepared using ethyl acetate and initially screened for anti-mycobacterial activity against Mycobacterium smegmatis using a single-concentration assay. Extracts exhibiting significant inhibition were further evaluated against Mycobacterium tuberculosis to determine minimum inhibitory concentrations (MICs) using a microdilution method. In addition, antibacterial activity against Staphylococcus aureus and Escherichia coli was assessed using a dot-blot assay to compare broader antimicrobial profiles. Cytotoxicity was evaluated using the NRK-52E cell line, and IC₅₀ values were calculated using GraphPad Prism software. The datasets include raw anti-mycobacterial screening data, MIC assay results against Mycobacterium tuberculosis, cytotoxicity measurements, and plate map layouts for microdilution experiments. The data are provided via a secure link for editorial review and will be made publicly available upon publication.