Percentage of aneuploid nuclei after chrysotile exposure and recovery.

Nuclear DNA content of control and chrysotile-treated (for 48 h) HK2 cells and allowed to recover in fiber-free medium for 2, 4 or 8 days was quantified, and nuclei with DNA content >5.1 C were considered aneuploid. Nuclei from mono-, bi- and multi-nucleated cells were quantified independently, and the aneuploid nuclei were identified. After 2 days of recovery, aneuploid nuclei were mainly in multinucleated cells, different to control cells and after long recovery periods – 4 and 8 days -, the aneuploid nuclei were predominantly in mononucleated cells. (P≤0.01: a x b, d x e, e x f, d x f; P