Hepatocyte nuclear factor 1ß gene regulation in E14.5 ureteric bud cells

HNF-1ß mutations are one of the most common single-gene mutations that underlie kidney developmental disease. Hepatocyte nuclear factor 1ß (HNF-1ß) is essential for kidney development, but its functions in ureteric bud (UB) branching morphogenesis are incompletely understood. We isolated E14.5 UB cells using fluorescence-activated cell sorting, and performed RNA-sequencing to compare gene expression in wild-type and HNF-1ß deficient UB cells. 1632 genes have significantly lower expression in the HNF-1ß deficient UB cells and 2223 genes have significantly higher expression in HNF-1ß deficient UB cells. Overall design: RNA-sequencing (RNA-seq) to compare gene expression in wild-type and HNF-1ß deficient E14.5 ureteric bud cells