File S1 - The Venom Gland Transcriptome of Latrodectus tredecimguttatus Revealed by Deep Sequencing and cDNA Library Analysis

Figure S1, The distribution of EST sequences in different GO categories. Figure S2, Distribution of the length of identified transcripts. Figure S3, Scatter plots of RPKM distribution of genes in the KEGG classes. Figure S4, The mapping of identified transcripts/proteins (marked as red) in the spliceosome pathway of KEGG database. Figure S5, The mapping of identified transcripts /proteins (marked as red) in the pathway of protein process in endoplasmic reticulum in KEGG database. Figure S6, Sequence characteristics of members in orphan families. Sequence characteristics of members in orphan families. The potential toxins, which haven’t homologue’s function annotations, are classified into orphan families including two groups: one comprises toxins predicted from Cys patterns, and the other is based on sequence homology with known toxins containing domains. Within Cys patterns, the char “#” represents any three amino acids other than Cys. For other toxins, the domain architectures were predicted by the SMART and Pfam servers [59,60]. The character “-F” appended to protein ID numbers indicates that these sequences are fragments but not full-length proteins. The abbreviations of domain names are as follow: EGF (SMART ID: SM00181); KU (SMART ID: SM00131), glyco_hydro_56 (Pfam ID: PF01630); crust_neurohorm (Pfam ID: PF01147). Figure S7, The abundance of toxin families in different functional categories. Bars represent toxin families clustered based on their functional characteristics. The sum of RPKM values for each class and category are labeled. Neurotoxins including the ANK superfamily, the SCP family and the lycotoxin family; Assistant toxins including theriditoxin family; Proteases including ctenitoxin family; Function unknown toxins including scorpion toxin like family and the orphan family. Figure S8, Phylogenomic trees for trypsin, scorpion toxin-like, lycotoxin, ctenitoxin, SCP family. Phylogenomic trees of trypsin, scorpion toxin-like, lycotoxin, ctenitoxin and SCP families. A. Ctenitoxin family; B. Trypsin family; C. Scorpion toxin-like family; D. SCP family; E. Lycotoxin family. The members of family and their homologues from other spiders are colored as blue and red on branches. For spider species that have transcriptomic data were highlighted by a green line. Figure S9, Phylogenetic tree of ANK superfamily toxins and their homologues from other 45 species. Phylogenetic tree of ANK superfamily toxins and their homologues from other 45 species. Color code: pink for α-LTX-Lt1a family1; blue for α-LTX-Lt1a family2; green for δ-LIT-Lt1a family; red for α-LIT-Lt1a family; brown for ANK family. All phylogeny analyses are performed with MEGA 5.2 using Maximum Likelihood algorithm and 1000 bootstrap tests. The numbers on the branches are the supporting percentages of 1000 bootstrap tests. Table S1, RPKM distribution in the top ten of three GO namespaces. Table S2, RPKM statistics of Ion channel in Latrodectus tredecimguttatus. Table S3, The statistics of RPKM in KEGG pathway superclass. Table S4, The RPKM list of sub-classes of the “Genetic information processing” category in KEGG database. Table S5, List of toxins identified by sequence analyses. Table S6, Known ion channel toxins in five venomous species. Table S7, Full names/abbreviations’ and taxonomic classification of 18 species in phylogenetic analysis. Table S8, Full names/abbreviations and taxonomic classification of 54 arthropod species. Table S9, Full names/abbreviations and taxonomic classification of species shown in Figure S8. (PDF)