Monolayer cells transcriptome of human embryonic stem cell differentiation

We examined the toxic effects of environmental pollutants on embryonic stem cell induced monolayer differentiation by RNA sequencing. RNA extraction was performed on monolayer cells exposed to multiple compounds during monolayer differentiation. The toxic effect of the compound on early embryonic development was obtained by analyzing the difference in RNA expression. Our study provides data closer to the toxic effects of human exposure to neonicotinoids, organophosphate esters and synthetic phenolic antioxidants. Overall design: The differences of RNA expression in monolayer cells after exposure to different compounds were examined.